Microspectrophotometry of single rhabdoms in the retina of the honeybee drone (Apis mellifera male)
نویسندگان
چکیده
The relative absorption spectra of the bistable photopigment of single rhabdoms from the dorsal region of the retina of the honeybee drone were obtained using slices of retina fixed in glutaraldehyde; less accurate measurements on unfixed tissue gave difference spectra that were similar to those for fixed retinae. The method used was based on measurements of absorbance changes during saturating adaptations of the visual pigment to different monochromatic lights. It is similar to previous methods based on measurements of difference spectra amplitudes, but is simpler to use and more accurate. The predominant pigment has states that absorb maximally at 446 (rhodopsin) and 505 nm (metarhodopsin). In addition, there is a small amount of another pigment whose two states absorb maximally at approximately 340 (UV) and 460 nm.
منابع مشابه
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The first step in fundamental breeding of any living organism is the determination of its genetic make-up. To investigate the genetic make-up of the European honeybee (Apis mellifera L.) in Iran, some research attempts were made during the years 1992-1996 in which the bee's relationship with some exotic races were determined through the application of morphological methods. For this purpose 130...
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ورودعنوان ژورنال:
- The Journal of General Physiology
دوره 82 شماره
صفحات -
تاریخ انتشار 1983